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signal stain boost detection reagent  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc signal stain boost detection reagent
    Signal Stain Boost Detection Reagent, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/signal+stain+boost/pm41712409-72-18-23
    Average 86 stars, based on 1 article reviews
    signal stain boost detection reagent - by Bioz Stars, 2026-10
    86/100 stars

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    Related Articles

    Incubation:

    Article Title: Critical role for non‑GAP function of Gαs in RGS1‑mediated promotion of melanoma progression through AKT and ERK phosphorylation.
    Article Snippet: Antibody of RGS1 (cat. no. PA5‐29579; Thermo Fisher Scientific, Inc., Waltham, MA, USA) was diluted according to the manufacturer instructions and the sections were incubated overnight at 4 ̊C. .. Then, the sections were washed three times, 5 min each, with TBS-T and incubated for 1 h at room temperature with Signal Stain Boost (Cell Signaling Technology, Inc., Danvers, MA, USA). ..

    Article Title: Essential role of NLRC5 in cancer immune surveillance and cancer immunoediting
    Article Snippet: .. Slides were incubated overnight at 4°C with a rat monoclonal antibody against mouse CD3 (ThermoFisher scientific, Cat #14-0032-81) diluted in blocking buffer, washed and then incubated with horseradish peroxidase (HRP)-conjugated secondary Ab for 1 h. After thorough washing, slides were incubated in 30-40 μL of signal stain boost (SignalStain ® Boost, Cell Signaling Technology, Cat # 8125) for 30 minutes at room temperature. .. Slides were incubated overnight at 4°C with a rat monoclonal antibody against mouse CD3 (ThermoFisher scientific, Cat #14-0032-81) diluted in blocking buffer, washed and then incubated with horseradish peroxidase (HRP)-conjugated secondary Ab for 1 h. After thorough washing, slides were incubated in 30-40 μL of signal stain boost (SignalStain Boost, Cell Signaling Technology, Cat # 8125) for 30 minutes at room temperature.

    Article Title: Genetic and pharmacological targeting of nicotinic acetylcholine receptor action blocks tumor progression in mouse models of breast cancer.
    Article Snippet: .. Tumor tissues resected upon harvest were embedded in formalin, 10 μm sections, subjected to antigen retrieval with Signal Stain Citrate Unmasking solution (Cell Signaling Technology; #14746), incubated with anti-CD11c antibody (Cell Signaling Technology; #97585S), and detected with a horseradish peroxidase detection system, Signal Stain Boost, per the manufacturer’s protocol (Cell Signaling Technology; #8112). .. Tumor tissues resected upon harvest were embedded in formalin, 10 μm sections, subjected to antigen retrieval with Signal Stain Citrate Unmasking solution (Cell Signaling Technology; #14746), incubated with anti-CD11c antibody (Cell Signaling Technology; #97585S), and detected with a horseradish peroxidase detection system, Signal Stain Boost, per the manufacturer’s protocol (Cell Signaling Technology; #8112).

    Article Title: Parkin regulates mitophagy and mitochondrial function to protect against alcohol-induced liver injury and steatosis in mice
    Article Snippet: .. Sections were then washed and incubated with signal stain boost (Cell Signaling, 8114S) for 30 min and developed by use of diaminobenzidine substrate (Vector, SK4105). ..

    Article Title: Essential Role of NLRC5 in Cancer Immune Surveillance and Cancer Immunoediting.
    Article Snippet: A key mechanism of tumour immune escape from CD8+ cytotoxic T lymphocytes occurs via downregulation of NLRC5, an IFNγinduced transcriptional activator of MHC classI.. As NLRC5 deficiency does not abrogate CD8+ T cell development, we investigated whether NLRC5dependent antitumour immune mechanisms are required for immune surveillance.. We studied the development of 3methylcholanthrene (MCA)induced endogenous fibrosarcoma in Nlrc5−/− mice with Nlrc5+/+ and Rag1−/− mice serving as controls.

    Article Title: Epithelial Regeneration After Doxorubicin Arises Primarily From Early Progeny of Active Intestinal Stem Cells
    Article Snippet: .. Sections were incubated with rabbit anti-GFP (1:2000, ab183734) in Signal Stain Antibody Diluent (Cell Signaling Technology, Danvers, MA; #8112) for 1 hour at room temperature, followed by Signal Stain Boost (Cell Signaling Technology; #8114) for 30 minutes at room temperature. .. Fluorescein reagent (PerkinElmer, Waltham, MA; NEL741001KT) was applied to the sections for 10 minutes prior to the stripping procedure.

    Staining:

    Article Title: Critical role for non‑GAP function of Gαs in RGS1‑mediated promotion of melanoma progression through AKT and ERK phosphorylation.
    Article Snippet: Antibody of RGS1 (cat. no. PA5‐29579; Thermo Fisher Scientific, Inc., Waltham, MA, USA) was diluted according to the manufacturer instructions and the sections were incubated overnight at 4 ̊C. .. Then, the sections were washed three times, 5 min each, with TBS-T and incubated for 1 h at room temperature with Signal Stain Boost (Cell Signaling Technology, Inc., Danvers, MA, USA). ..

    Article Title: Essential role of NLRC5 in cancer immune surveillance and cancer immunoediting
    Article Snippet: .. Slides were incubated overnight at 4°C with a rat monoclonal antibody against mouse CD3 (ThermoFisher scientific, Cat #14-0032-81) diluted in blocking buffer, washed and then incubated with horseradish peroxidase (HRP)-conjugated secondary Ab for 1 h. After thorough washing, slides were incubated in 30-40 μL of signal stain boost (SignalStain ® Boost, Cell Signaling Technology, Cat # 8125) for 30 minutes at room temperature. .. Slides were incubated overnight at 4°C with a rat monoclonal antibody against mouse CD3 (ThermoFisher scientific, Cat #14-0032-81) diluted in blocking buffer, washed and then incubated with horseradish peroxidase (HRP)-conjugated secondary Ab for 1 h. After thorough washing, slides were incubated in 30-40 μL of signal stain boost (SignalStain Boost, Cell Signaling Technology, Cat # 8125) for 30 minutes at room temperature.

    Article Title: Genetic and pharmacological targeting of nicotinic acetylcholine receptor action blocks tumor progression in mouse models of breast cancer.
    Article Snippet: .. Tumor tissues resected upon harvest were embedded in formalin, 10 μm sections, subjected to antigen retrieval with Signal Stain Citrate Unmasking solution (Cell Signaling Technology; #14746), incubated with anti-CD11c antibody (Cell Signaling Technology; #97585S), and detected with a horseradish peroxidase detection system, Signal Stain Boost, per the manufacturer’s protocol (Cell Signaling Technology; #8112). .. Tumor tissues resected upon harvest were embedded in formalin, 10 μm sections, subjected to antigen retrieval with Signal Stain Citrate Unmasking solution (Cell Signaling Technology; #14746), incubated with anti-CD11c antibody (Cell Signaling Technology; #97585S), and detected with a horseradish peroxidase detection system, Signal Stain Boost, per the manufacturer’s protocol (Cell Signaling Technology; #8112).

    Article Title: Parkin regulates mitophagy and mitochondrial function to protect against alcohol-induced liver injury and steatosis in mice
    Article Snippet: .. Sections were then washed and incubated with signal stain boost (Cell Signaling, 8114S) for 30 min and developed by use of diaminobenzidine substrate (Vector, SK4105). ..

    Article Title: Essential Role of NLRC5 in Cancer Immune Surveillance and Cancer Immunoediting.
    Article Snippet: A key mechanism of tumour immune escape from CD8+ cytotoxic T lymphocytes occurs via downregulation of NLRC5, an IFNγinduced transcriptional activator of MHC classI.. As NLRC5 deficiency does not abrogate CD8+ T cell development, we investigated whether NLRC5dependent antitumour immune mechanisms are required for immune surveillance.. We studied the development of 3methylcholanthrene (MCA)induced endogenous fibrosarcoma in Nlrc5−/− mice with Nlrc5+/+ and Rag1−/− mice serving as controls.

    Article Title: Epithelial Regeneration After Doxorubicin Arises Primarily From Early Progeny of Active Intestinal Stem Cells
    Article Snippet: .. Sections were incubated with rabbit anti-GFP (1:2000, ab183734) in Signal Stain Antibody Diluent (Cell Signaling Technology, Danvers, MA; #8112) for 1 hour at room temperature, followed by Signal Stain Boost (Cell Signaling Technology; #8114) for 30 minutes at room temperature. .. Fluorescein reagent (PerkinElmer, Waltham, MA; NEL741001KT) was applied to the sections for 10 minutes prior to the stripping procedure.

    Blocking Assay:

    Article Title: Essential role of NLRC5 in cancer immune surveillance and cancer immunoediting
    Article Snippet: .. Slides were incubated overnight at 4°C with a rat monoclonal antibody against mouse CD3 (ThermoFisher scientific, Cat #14-0032-81) diluted in blocking buffer, washed and then incubated with horseradish peroxidase (HRP)-conjugated secondary Ab for 1 h. After thorough washing, slides were incubated in 30-40 μL of signal stain boost (SignalStain ® Boost, Cell Signaling Technology, Cat # 8125) for 30 minutes at room temperature. .. Slides were incubated overnight at 4°C with a rat monoclonal antibody against mouse CD3 (ThermoFisher scientific, Cat #14-0032-81) diluted in blocking buffer, washed and then incubated with horseradish peroxidase (HRP)-conjugated secondary Ab for 1 h. After thorough washing, slides were incubated in 30-40 μL of signal stain boost (SignalStain Boost, Cell Signaling Technology, Cat # 8125) for 30 minutes at room temperature.

    Article Title: Essential Role of NLRC5 in Cancer Immune Surveillance and Cancer Immunoediting.
    Article Snippet: A key mechanism of tumour immune escape from CD8+ cytotoxic T lymphocytes occurs via downregulation of NLRC5, an IFNγinduced transcriptional activator of MHC classI.. As NLRC5 deficiency does not abrogate CD8+ T cell development, we investigated whether NLRC5dependent antitumour immune mechanisms are required for immune surveillance.. We studied the development of 3methylcholanthrene (MCA)induced endogenous fibrosarcoma in Nlrc5−/− mice with Nlrc5+/+ and Rag1−/− mice serving as controls.

    other:

    Article Title: Phosphorylation of the MNK1 substrate eIF4E is not required for response to acute pancreatitis.
    Article Snippet: Protein concentration was estimated using the Bradford method (Protein Assay kit, Biorad).



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